PinkMonkey Online Study Guide-Biology
8.7 The Genetic Code
Some historical events
1954 |
Gamow : Suggested the triplet genetic code. |
1958 |
Crick : DNA determines the sequence of amino acids in
a polypeptide (Central dogma of molecular biology).
|
1961 |
Crick et al : Provided direct evidence for triplet code. |
1966 |
Crick : Proposed the wobble hypothesis for the genetic code. |
1967 |
Komberg and Sinsheimer : Synthesized single-stranded DNA in a bacteriophage. |
1970 |
Khorana : Synthesized an artificial gene from DNA nucleotides. |
The genetic code : DNA is genetic material
and contains genetic information. The expression of a gene takes
place through specific enzymes. Each gene produces a specific (one-gene
one-protien hypothesis). In other words, formation of each specific
protein is controlled by a particular gene. A gene is (almost always)
a segment of DNA strand and so, the information for the formation
of a protein is contained in the DNA strand.
Further, each protein is a long polypeptide chain molecule formed by joining amino acid molecules. From the cell pool, only 20 different types of amino acids are used for protein synthesis.
The sequence of the nitrogen bases in the DNA determines
the sequence of amino acids in a protein molecule through the mRNA. This
sequence is copied down by the mRNA (transcription). It is present on
the mRNA strand in the form of coded language (cyptogram or mRNA
language or genetic code). The mRNA bases (A, U, C and G) serve
as the four alphabets of the coded language.
Codon : The smallest sequence of the nitrogen
bases (nucleotides) on the mRNA which can specify one amino acid is called
a codon . Each codon consists of three successive bases on the
mRNA.
Why should each codon in the genetic code consist
of 3 bases (triplet codon) and not of one base each or 2 bases each?
This is because there are 20 different amino acids which can be used in
the synthesis of proteins in the cells. There must be at least one specific
codon for each amino acid. Thus, there has to be at least 20 different
codons in the genetic code. There are only four bases. A minimum of 3
bases per codon is necessary to have (a minimum of) 20 codes.
The wobble hypothesis (Crick, 1966) : The anticodon
on tRNA is complementary to the codon on the mRNA as per the A = U, G
= C base pairing rule. However, it has been observed that the 3rd base
position may vary and yet still code for the same amino acid. For example,
both codons TTA and TTG code for the amino acid, leucine. Thus, the third
position is called the wobble position.
Thus, Crick’s (1966) wobble hypothesis explains the degeneracy of the genetic code at the third position of the codon.
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